AustralieFrV1, Main, Exploration, bibRecord, 002137

DEFECTIVE KERNEL1 (DEK1) Regulates Cell Walls in the Leaf Epidermis.

Identifieur interne : 002137 ( Main/Exploration ); précédent : 002136; suivant : 002138

DEFECTIVE KERNEL1 (DEK1) Regulates Cell Walls in the Leaf Epidermis.

Auteurs : Dhika Amanda [Australie] ; Monika S. Doblin [Australie] ; Roberta Galletti [Australie] ; Antony Bacic [Australie] ; Gwyneth C. Ingram [Australie] ; Kim L. Johnson [Australie]

Source :

Plant physiology [ 1532-2548 ] ; 2016.

RBID : pubmed:27756823

Descripteurs français

English descriptors

KwdEn :
- Arabidopsis (cytology), Arabidopsis (genetics), Arabidopsis (metabolism), Arabidopsis (ultrastructure), Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), Calpain (genetics), Calpain (metabolism), Cell Wall (metabolism), Cell Wall (ultrastructure), Epitopes (metabolism), Gene Expression Regulation, Plant, Genes, Plant, Kinetics, Models, Biological, Pectins (metabolism), Phenotype, Plant Development (genetics), Plant Epidermis (cytology), Plant Epidermis (metabolism), Plant Epidermis (ultrastructure), RNA, Messenger (genetics), RNA, Messenger (metabolism), Real-Time Polymerase Chain Reaction.
MESH :
- chemical , genetics : Arabidopsis Proteins, Calpain, RNA, Messenger.
- cytology : Arabidopsis, Plant Epidermis.
- genetics : Arabidopsis, Plant Development.
- metabolism : Arabidopsis, Arabidopsis Proteins, Calpain, Cell Wall, Epitopes, Pectins, Plant Epidermis, RNA, Messenger.
- ultrastructure : Arabidopsis, Cell Wall, Plant Epidermis.
- Gene Expression Regulation, Plant, Genes, Plant, Kinetics, Models, Biological, Phenotype, Real-Time Polymerase Chain Reaction.

Abstract

The plant epidermis is crucial to survival, regulating interactions with the environment and controlling plant growth. The phytocalpain DEFECTIVE KERNEL1 (DEK1) is a master regulator of epidermal differentiation and maintenance, acting upstream of epidermis-specific transcription factors, and is required for correct cell adhesion. It is currently unclear how changes in DEK1 lead to cellular defects in the epidermis and the pathways through which DEK1 acts. We have combined growth kinematic studies, cell wall analysis, and transcriptional analysis of genes downstream of DEK1 to determine the cause of phenotypic changes observed in DEK1-modulated lines of Arabidopsis (Arabidopsis thaliana). We reveal a novel role for DEK1 in the regulation of leaf epidermal cell wall structure. Lines with altered DEK1 activity have epidermis-specific changes in the thickness and polysaccharide composition of cell walls that likely underlie the loss of adhesion between epidermal cells in plants with reduced levels of DEK1 and changes in leaf shape and size in plants constitutively overexpressing the active CALPAIN domain of DEK1. Calpain-overexpressing plants also have increased levels of cellulose and pectins in epidermal cell walls, and this is correlated with the expression of several cell wall-related genes, linking transcriptional regulation downstream of DEK1 with cellular effects. These findings significantly advance our understanding of the role of the epidermal cell walls in growth regulation and establish a new role for DEK1 in pathways regulating epidermal cell wall deposition and remodeling.

DOI: 10.1104/pp.16.01401
PubMed: 27756823

Affiliations:

Le document en format XML

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<term>Arabidopsis Proteins (genetics)</term>
<term>Arabidopsis Proteins (metabolism)</term>
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<term>Calpain (metabolism)</term>
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<term>Cell Wall (ultrastructure)</term>
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<term>Genes, Plant</term>
<term>Kinetics</term>
<term>Models, Biological</term>
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<term>Plant Development (genetics)</term>
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<term>Plant Epidermis (metabolism)</term>
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<term>RNA, Messenger (metabolism)</term>
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<term>Arabidopsis (métabolisme)</term>
<term>Arabidopsis (ultrastructure)</term>
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<term>Épiderme végétal (métabolisme)</term>
<term>Épiderme végétal (ultrastructure)</term>
<term>Épitopes (métabolisme)</term>
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<front><div type="abstract" xml:lang="en">The plant epidermis is crucial to survival, regulating interactions with the environment and controlling plant growth. The phytocalpain DEFECTIVE KERNEL1 (DEK1) is a master regulator of epidermal differentiation and maintenance, acting upstream of epidermis-specific transcription factors, and is required for correct cell adhesion. It is currently unclear how changes in DEK1 lead to cellular defects in the epidermis and the pathways through which DEK1 acts. We have combined growth kinematic studies, cell wall analysis, and transcriptional analysis of genes downstream of DEK1 to determine the cause of phenotypic changes observed in DEK1-modulated lines of Arabidopsis (Arabidopsis thaliana). We reveal a novel role for DEK1 in the regulation of leaf epidermal cell wall structure. Lines with altered DEK1 activity have epidermis-specific changes in the thickness and polysaccharide composition of cell walls that likely underlie the loss of adhesion between epidermal cells in plants with reduced levels of DEK1 and changes in leaf shape and size in plants constitutively overexpressing the active CALPAIN domain of DEK1. Calpain-overexpressing plants also have increased levels of cellulose and pectins in epidermal cell walls, and this is correlated with the expression of several cell wall-related genes, linking transcriptional regulation downstream of DEK1 with cellular effects. These findings significantly advance our understanding of the role of the epidermal cell walls in growth regulation and establish a new role for DEK1 in pathways regulating epidermal cell wall deposition and remodeling.</div>
</front>
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DEFECTIVE KERNEL1 (DEK1) Regulates Cell Walls in the Leaf Epidermis.

DEFECTIVE KERNEL1 (DEK1) Regulates Cell Walls in the Leaf Epidermis.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur les relations entre la France et l'Australie
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